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Honestly, it'd be funnier if the guy who did it is someone who has like, no other morals. He'll punch a pregnant lady, he'll kill an old woman. Childern, however? No.
I would love for there to be a tmnt villain who at first is just going after them like every other one does, but then after having a Realization that they are Actual Teenagers it's revealed that the dude's got a strict Don't Hurt Kids line to their moral code.
Thing is, they still keep trying to do whatever villainy they first aimed for it's just that, instead of directly fighting the gang, they keep creating increasingly elaborate non-lethal traps to keep the guys busy while the villain does their plot and just bails if the guys show up early.