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To be honest, reading this made me think that Timmy will be the main villain in season 2. Think about it. Timmy doesn't even remember anything about Cosmo or Wanda anymore. But in Channel Chasers, what if he remembered them when his kids showed him the picture of him and his fairies. It would make him try to find them and reunite in a bad way.
Chronologically, it was episode 16. It would be ironic if some kind of disaster will happen in season 2, episode 16. Yes, that would be very interesting.
Also, the episode is called "Cookie's Court". So, Cookie's return? Considering that she is in prison at the moment, it means that someone will help her get out. Now imagine that this someone is NOT Irep. Which, in principle, would be too expected, firstly. Secondly, Irep has already existed and if he appears in the second season, he will most likely be more of a "chaotic neutral" character who can and will do something bad, but will never go too far(not this time). So it really will most likely not be Irep. This someone will most likely act alone. And this "mysterious villain" must have a special reason (his own benefit) to release Cookie. Also, for some reason I think that in this case, Cookie will not be the main villain (could she be manipulated by the main villain of the 2nd season)?
It is also possible that it could be someone we already know, which will be exactly that "plot twist". What are your guesses?